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Un biorreactor o fermentador es un recipiente en el cual se ponen en presencia una de otra a fin de que se desarrolle el proceso microbiol Los microorganismos o c. Las condiciones ambientales de un biorreactor tales como flujo de gases (por ejemplo, ox. Para evitar esto, el biorreactor debe ser f. Pueden ser refrigerados con una chaqueta externa o, para recipientes sumamente grandes, con serpentines internos. En un proceso de aer. La transferencia de ox. Sin embargo, existen l. Los organismos superiores tambi. Un biorreactor es por tanto un dispositivo biotecnol. Externamente el biorreactor es la frontera que protege ese cultivo del ambiente externo: contaminado y no controlado. El biorreactor debe por tanto suministrar los controles necesarios para que la operaci. Aunque se puede describir de manera similar a la qu. Por eso, lo primero que se define en el dise. El conjunto biorreactor- sistema de cultivo debe cumplir con los siguientes objetivos: Mantener las c. En los reactores biol. Biography & Memoir; Business & Leadership; Fiction. Al operar un biorreactor en una determinada categor. El metabolismo define los par. Pese a su versatilidad, sus mayores representantes son microorganismos que presentan relaciones par. Sus principales grupos est. Son microorganismos aer. Los biorreactores para c. Actualmente, el cultivo de protoplastos no es muy com. Para suministrar esa proximidad y el soporte necesario, los dise. Una forma de hacerlo es incorporar un lecho fluidizado formado por cantidad de microesferas acarreadoras hechas de material cer. Los cultivos celulares animales, por la delicada naturaleza de las membranas plasm. Esto es especialmente cr. El medio de cultivo fluye en contrasentido desde el exterior del reactor o a trav. Para solventar el problema de la escasa transferencia de masa (nutrientes y OD) dentro de la fibra hueca, un dise. Un lecho empacado es una matriz de soporte s. Un biorreactor con este prop. Debido a que un sustrato enzim. Muchas veces el medio de cultivo, adem. Existen tres modos de cultivo aunados a tres modos b. Antes de modelar en ecuaciones un dise. Eso es, hacer un balance para . De esta forma el primer balance es: Balance general biomasa. A partir del balance general, debe establecerse un balance general para cada componente i. Para modelar el comportamiento de la biomasa del cultivo en el estado estacionario (EE) adem. Esto se conoce como quimioest. La ec. 3 que se conoce como ecuaci. Y en el balance de materia se anula el t. El tiempo que dura una operaci. Una vez cargado el biorreactor e inoculado el medio de cultivo, la concentraci. Por este motivo, el tiempo que dura el cultivo dentro de un biorreactor con un modo de operaci. El caudal de salida F contiene c. Al alimentar con medio fresco (So) el caudal de entrada (F1), la biomasa (Xo) y el producto (Po) ser. Despejando: Ni = Nio (1 - Xi) . Donde: t = tiempo de residencia del cultivo dentro del biorreactor; . El tiempo espacial se obtiene dividiendo el volumen de reactor (V) entre el caudal volum. Para iniciar un cultivo alimentado (fed- batch) son validas las mismas consideraciones que se hicieron para el cultivo continuo; pero, se inicia la alimentaci. Bajo estas condiciones, la ecuaci. No obstante esto ocasionar. Para buscar el equilibrio se dise. El balance general de masa que se aplica a un volumen de control (diferencial de volumen) para un componente i en un sistema de flujo semicontinuo es: Entra - Sale - Desaparece = Acumula . Resolviendo la integral obtenemos: V/Fio = . Cuando se requiere una expresi. Sustituyendo: V/Fio = V/Qi. Cio = . En un biorreactor discontinuo, t representa el tiempo de cultivo tc y es igual a la duraci. En un biorreactor semicontinuo (de flujo) el tiempo t corresponde al equivalente para que la conversi. Es por eso que en un biorreactor de flujo el tiempo t se llama tambi. Afortunadamente, es posible analizar casos particulares, haciendo algunas suposiciones. En el caso de tomar en cuenta . Note que antes de la fase exponencial existe una fase de retardo durante la cual, la concentraci. Normalmente, la fase de retardo no es deseable, tanto por la p. Como condiciones de idealizaci. Para completar el esquema de dise. El sistema de agitaci. Finalmente, el tanque debe poseer un intercambiador de calor formado por una camisa por la que circule agua, para poder controlar la temperatura del cultivo y evitar que este muera o sufra un estr. Debido a que, a mayor volumen de cultivo, tambi. Ver esquema representado en la figura. Al igual que en el dise. En los biorreactores de levantamiento por aire o . El KLa y por lo tanto el grado de transferencia de ox. En general, disminuyen el KLa: la viscosidad y el volumen del l. Un sistema de aireaci. Y tres partes de control: control de flujo aire; control de presi. Una segunda e importante caracter. Existen dos tipos de dise. El compresor de diafragma resulta adecuado para oxigenar vol. Aunque su rango de acci. Para sistemas mayores (industriales) debe dise. Las membranas microporo que filtran el aire tienen un punto de burbuja que es la presi. El sistema consta de dos partes mec. Alternativamente y si el sistema es peque. Se debe conocer la r. KLa; el valor de r. Ambas razones pueden medirse regularse con un controlador OUR/SOUR de uso comercial. Una probeta o electrodo OD es un sensor que mide la concentraci. Similarmente, una probeta o electrodo BOD mide la concentraci. Los fermentadores suelen operar en modo semicontinuo; por cuanto, debe alimentarse una l. Las espumas modifican la acidez del medio de cultivo ya que, cambian el p. H del medio de cultivo y por tanto, debe regularse y controlarse la acidez del medio adecuadamente. Adicionalmente, la turbidez y la cantidad de espuma, deben mantenerse en niveles adecuados para evitar p. Este debe ser de acero inoxidable y su di. Un sistema de control de acidez consta de: Dos subsistemas mec. El intercambiador de calor de caso y tubos es el m. La temperatura afecta a las c. El contenedor es la frontera f. Un tanque contenedor o cuerpo del biorreactor se debe construir en acero inoxidable austen. De esta forma, es posible escalar; es decir cambiar de dimensi. Las principales relaciones adimensionales que se utilizan en tanques agitados son: la raz. En la figura aparece como . Finalmente, C es la altura de piso del agitador . Su longitud depende de la profundidad del contenedor (tanque). Acople del Eje Transmisor: ajusta y fija al motor, el eje transmisor de potencia. Existen dos tipos de acople: Acople- adaptador de tipo taladro: el puerto de entrada se acopla al eje del motor por fijaci. El puerto de salida es un dispositivo que se adapta a varios di. El puerto de salida es un dispositivo que . Como se observa en la fotograf. Cada dispositivo de anclaje o sujetador tambi. Algunos puertos tienen dos di. Dentro de lo posible se recomienda el uso de sellos flexibles ya que amortiguan mejor las vibraciones mec. Existen ejes en los cuales ya vienen incorporadas hojas o aspas de agitaci. Por eso se recomienda impulsores de flujo axial para cultivar c. Estas velocidades son muy altas para los sistemas biol. La velocidad de rotaci. Usualmente se acopla a la salida de eje del rotor una caja de reducci. Adicionalmente se coloca un control de velocidad que puede ser anal. Caso contrario ocurre cuando Dd es muy peque. Para que la Pf se transmita a todo el volumen de operaci. La mejor forma de combinar positivamente estos efectos hidrodin. A esto se le conoce como potencia . La segunda aumenta la potencia de mezclado y la potencia fluida pero tambi. El calor se transfiere por una combinaci. Uno de los mecanismos t. Aunque el mecanismo exacto de la conducci. La temperatura de salida del fluido fr. Un intercambiador con b > 7. Como se observa en la figura en los intercambiadores de calor compactos los dos fluidos fluyen en direcciones ortogonales entre s. Los tubos se disponen con sus ejes paralelos al eje del casco; la transferencia de calor tiene lugar, a medida que el fluido que se desea calentar o enfriar, se mueve (fluye) por el interior de los tubos met. Microbiologia Industrial. ISBN 9. 78- 9. 87- 9. Biotecnologia. 5: Tecnolog. Los microorganismos o c. Las condiciones ambientales de un reactor biol. Para evitar esto, el reactor biol. Pueden ser refrigerados con una chaqueta externa o, para recipientes sumamente grandes, con serpentines internos. En los reactores biol. Los organismos superiores tambi. El sistema celular particular empleado. El funcionamiento de cualquier biorreactor depende de muchas funciones incluyendo. Esta es una clasificaci. En los reactores biol. Al operar un reactor biol. Los bioprocesos de cultivo y las fermentaciones est. El metabolismo define los par. La cantidad de aire necesario para la reacci. El aire de proceso se introduce en el fondo del tubo iscendente, en donde la flotaci. En la parte superior, la mayor. Las ventajas principales de los biorreactores de ele. Las desventajas principa. Aunque los requerimientos de energ. Sin embargo, tan pronto como no se cumpl. En tabla algunos datos de mezclado para columnas de burbujeo y brazos lazos de aire de distintos tama. Puede verse que para tener una homogeneidad n necesario recorrer seis veces el reactor despu. Esto a su vez se debe a que la diferencia de pe la fuerza impulsora causada por la retenci. En funcionamiento metab. En este proceso se usa substrato para producir prote. En Rumania se encuentra ojeaw instalaci. En este proceso se emplean cuatro reactores de coknaa j bujas cada una con una volumen de 1 2. Si la densidad de part. Esto tiene la ventaja de que se puede usar una densidad de part. La idea inicial de cultivar c. Este tipo de resina se us. La Toxicidad del soporte puede causar sirgas fases de retardo, la muerte de las c. Las fibras huecas se pueden hacer de acetato de celulosa con una matriz de pared uniforme, . Estas crisis huecas tienen una superficie altamente porosa de casi 7. La velocidad de transferencia de 0. El KLa y por lo tanto el grado de transferencia de ox. En general, disminuyen el KLa: la viscosidad y el volumen del l. El sistema de aireaci. Un sistema de aireaci. Y tres partes de control: control de flujo aire; control de presi. El sistema consta de dos partes mec. Se debe conocer la r. KLa; el valor de r. Ambas razones pueden medirse, regularse con un controlador OUR/SOUR. Una probeta o electrodo OD es un sensor que mide la concentraci. Similarmente, una probeta o electrodo BOD mide la concentraci. En ambos casos, el material de su construcci. La transferencia de ox. Primeramente el oxigeno debe viajar a trav. El proceso completo es impulsado por la diferencia entre la concentraci. Para conocer la rapidez total de transferencia de oxigeno en un recipiente, se tiene que determinar el . Esta es una cantidad dif. La siguiente figura es un esquema de dicho reactor. Los recipientes para experimentos de la tecnologia son dise. El agitador puede ser accionado desde la parte superior o desde la parte inferior. Todos los tanques est. Usualmente se instalan cuatro mamparas con una anchura a di. Sin embargo, la suspensi. La finalidad de la agitaci. Consta de varias paletas sujetas a un eje central. Las turbinas con aspas planas dan un flujo radial, como se muestra en la figura 1. Cuando est. Otro grupo usado para caracterizar la mezcla en un recipiente, el cual toma en cuenta efectos gravitacionales, es el n. Debe quedar establecido que la mayor parte del trabajo sobre agitaci. Sin embargo, las interacciones con el tubo rociador son muy complejas que con las turbinas de disco y se necesita m. En la tabla 1. 4. Puede verse que los agitadores de bombeo hacia arriba suspenden s. El acoplamiento del eje del motor es directo y el sellado es mediante un sello mec. El hilo puede terminar recto (aguja), en anillo (asa) o aplastado (esp. Ejemplo de siembra en un medio de cultivo. |
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